DNA

Part:BBa_K4889000:Design

Designed by: zhaoxia zhang   Group: iGEM23_Shanghai-MedX   (2023-08-24)


MST2 1-308


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 186
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 12
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 863


Design Notes

The Hippo pathway has been identified as a potential drug target for cancer. Furthermore, the MST2-STRN3 interaction was chosen as a target because of its importance in the Hippo pathway and previous studies in the laboratory. In this project, we want to find out if the FDA drug can disrupt the MST2-STRN3 interaction to activate the Hippo pathway. First, we need to express these proteins.


Source

MST2 1-308 from Homo sapiens are synthesized.

References